ROP2 from Toxoplasma gondii: a virulence factor with a protein-kinase fold and no enzymatic activity.
Structure
; 17(1): 139-46, 2009 Jan 14.
Article
en En
| MEDLINE
| ID: mdl-19141290
ABSTRACT
The ROP2 protein and its paralogs are important virulence factors secreted into the host cell by the parasite Toxoplasma gondii. Here we describe the crystal structure of a large and soluble domain of mature ROP2, representative of the ROP2-like protein family. This is a structure of a protein-kinase fold that is devoid of catalytic residues and does not bind ATP. Various structural extensions constitute a signature of this protein family and act to maintain the protein kinase in an open conformation. Our ROP2 structure rules out a previous structural model of attachment of ROP2-like proteins to the parasitophorous vacuole membrane. We propose an alternative mode of membrane attachment implicating basic and amphiphatic helices present in the flexible N terminus of ROP2.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Toxoplasma
/
Proteínas Protozoarias
/
Proteínas de la Membrana
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Structure
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2009
Tipo del documento:
Article