Synthetic peptides of Venezuelan equine encephalomyelitis virus E2 glycoprotein. III. Identification of a protective peptide derived from the carboxy-terminal extramembranal one-third of the protein.
Virology
; 185(2): 840-2, 1991 Dec.
Article
en En
| MEDLINE
| ID: mdl-1962453
ABSTRACT
To complete our analysis of the E2 glycoprotein of Venezuelan equine encephalomyelitis (VEE) virus, we prepared six synthetic peptides corresponding to the extramembranal carboxy-terminal one-third of the protein. NIH-Swiss mice were immunized with the peptides, and antipeptide and antiviral titers were determined by enzyme-linked immunosorbent assay (ELISA). Challenge studies revealed that peptide 13 (amino acids 241-265) protected 60-70% of virus-challenged mice. Although the other peptides generally elicited antipeptide ELISA titers but no or low antiviral titers and did not protect mice, significant E2 reactivity was found in immunoblots. These results provide the first direct evidence that much of the E2 carboxy-terminal domain is cryptic in the VEE virion, even when virus was bound to polystyrene ELISA plates.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Vacunas Virales
/
Proteínas del Envoltorio Viral
/
Virus de la Encefalitis Equina Venezolana
/
Encefalomielitis Equina Venezolana
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
Virology
Año:
1991
Tipo del documento:
Article