Yak interferon-alpha loaded solid lipid nanoparticles for controlled release.
Res Vet Sci
; 88(1): 148-53, 2010 Feb.
Article
en En
| MEDLINE
| ID: mdl-19647842
ABSTRACT
To explore the potential of a novel animal interferon formulation for controlled release, the yak interferon-alpha (IFN-alpha) glutathione S-transferase (GST) fusion protein was expressed in Escherichia coli (E. coli) and the purified recombinant IFN-alpha was encapsulated into solid lipid nanoparticles (SLN) by double emulsion solvent evaporation (w/o/w) method. The particle size and zeta potential of IFN-alpha-loaded SLN were 124.2+/-10.2 nm and -11.2+/-0.6 mV. The encapsulation efficiency of IFN-alpha and loading capacity of the SLN were 83.7+/-4.5% and 1.73+/-0.15%, respectively. In vitro release study and antiviral assay demonstrated that the IFN-alpha released from the SLN in a 16-day period exhibited antiviral activity in Madin-Darby bovine kidney (MDBK) cells against vesicular stomatitis virus (VSV), and showed a release pattern of an initial burst release followed by a sustained and slow release. Cytotoxicity assay in cell culture demonstrated that the SLN were not toxic. The results of this exploratory study suggest that the IFN-alpha-loaded SLN could be a useful formulation for controlled release in veterinary therapeutics.
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1
Base de datos:
MEDLINE
Asunto principal:
Antivirales
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Interferón-alfa
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Preparaciones de Acción Retardada
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Nanopartículas
Idioma:
En
Revista:
Res Vet Sci
Año:
2010
Tipo del documento:
Article