Effect of phosphate on aluminium-inhibited growth and signal transduction pathways in Coffea arabica suspension cells.
J Inorg Biochem
; 103(11): 1497-503, 2009 Nov.
Article
en En
| MEDLINE
| ID: mdl-19740543
ABSTRACT
In acid soils, aluminium (Al) toxicity and phosphate (Pi) deficiency are the most significant constraints on plant growth. Al inhibits cell growth and disrupts signal transduction processes, thus interfering with metabolism of phospholipase C (PLC), an enzyme involved in second messenger production in the cell. Using a Coffea arabica suspension cell model, we demonstrate that cell growth inhibition by Al toxicity is mitigated at a high Pi concentration. Aluminium-induced cell growth inhibition may be due to culture medium Pi deficiency, since Pi forms complexes with Al, reducing Pi availability to cells. Phosphate does not mitigate inhibition of PLC activity by Al toxicity. Other enzymes of the phosphoinositide signal transduction pathway were also evaluated. Aluminium disrupts production of second messengers such as inositol 1,4,5-trisphosphate (IP(3)) and phosphatidic acid (PA) by blocking PLC activity; however, phospholipase D (PLD) and diacylglycerol kinase (DGK) activities are stimulated by Al, a response probably aimed at counteracting Al effects on PA formation. Phosphate deprivation also induces PLC and DGK activity. These results suggest that Al-induced cell growth inhibition is not linked to PLC activity inhibition.
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Base de datos:
MEDLINE
Asunto principal:
Fosfatos
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Fosfolipasas de Tipo C
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Transducción de Señal
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Coffea
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Aluminio
Idioma:
En
Revista:
J Inorg Biochem
Año:
2009
Tipo del documento:
Article