Two factor reprogramming of human neural stem cells into pluripotency.
PLoS One
; 4(9): e7044, 2009 Sep 18.
Article
en En
| MEDLINE
| ID: mdl-19763260
ABSTRACT
BACKGROUND:
Reprogramming human somatic cells to pluripotency represents a valuable resource for the development of in vitro based models for human disease and holds tremendous potential for deriving patient-specific pluripotent stem cells. Recently, mouse neural stem cells (NSCs) have been shown capable of reprogramming into a pluripotent state by forced expression of Oct3/4 and Klf4; however it has been unknown whether this same strategy could apply to human NSCs, which would result in more relevant pluripotent stem cells for modeling human disease. METHODOLOGY AND PRINCIPALFINDINGS:
Here, we show that OCT3/4 and KLF4 are indeed sufficient to induce pluripotency from human NSCs within a two week time frame and are molecularly indistinguishable from human ES cells. Furthermore, human NSC-derived pluripotent stem cells can differentiate into all three germ lineages both in vitro and in vivo. CONCLUSIONS/SIGNIFICANCE:
We propose that human NSCs represent an attractive source of cells for producing human iPS cells since they only require two factors, obviating the need for c-MYC, for induction into pluripotency. Thus, in vitro human disease models could be generated from iPS cells derived from human NSCs.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Regulación de la Expresión Génica
/
Factores de Transcripción de Tipo Kruppel
/
Factor 3 de Transcripción de Unión a Octámeros
/
Lóbulo Frontal
/
Neuronas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2009
Tipo del documento:
Article