N-Glycosylation profiling of turtle egg yolk: expression of galabiose structure.
Carbohydr Res
; 345(3): 442-8, 2010 Feb 11.
Article
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| MEDLINE
| ID: mdl-20044081
ABSTRACT
To understand the roles of species-specific carbohydrates, systematic studies of interspecific glycan analyses are imperative. An extensive series of glycomics studies on approximately 180 kinds of bird eggs have demonstrated that 60-70% of the birds, which are closely related in phylogeny, express the alpha-Galp-(1-->4)-Galp structure on their egg glycoproteins. This prompted us to investigate the glycosylation profiles of eggs from an evolutionarily related organism, a sea turtle (reptilian). We performed N-glycosylation profiling of turtle egg yolk by using HPLC mapping in conjunction with mass spectrometric methods and thereby demonstrated that the alpha-Galp-(1-->4)-Galp groups are displayed on approximately 38% of total N-glycans. Our findings suggest that the ability to express the galabiose structure was acquired at an early stage of diversification in amniotes.
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MEDLINE
Asunto principal:
Tortugas
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Disacáridos
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Yema de Huevo
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Glicómica
Idioma:
En
Revista:
Carbohydr Res
Año:
2010
Tipo del documento:
Article