Molecular interactions in surface-assembled monolayers of short double-stranded DNA.

ABSTRACT

We present an experimental study of the energetics of repulsion between end-grafted fragments of double-stranded DNA. The absorption isotherm of thiolated DNA fragments has been measured as a function of DNA chain length as well as the salinity of the surrounding solution. The results are consistent with a simple excluded-volume model of the interaction between neighboring DNA strands.