Bioconversion of glycyrrhizinic acid in liquorice into 18-beta-glycyrrhetinic acid by Aspergillus parasiticus speare BGB.
Prikl Biokhim Mikrobiol
; 46(4): 462-6, 2010.
Article
en En
| MEDLINE
| ID: mdl-20873172
ABSTRACT
A filamentous fungi strain, Aspergillus parasiticus Speare BGB, producing beta-glucuronidase was screened to transform glycyrrhizinic acid (GL) in liquorice into 18-beta-glycyrrhetinic acid (GA). Under the following cultivate conditions in shake flask, 1% GL (purity 30%), medium capacity 40% of flask, the initial pH value at 4.5, cultivate temperature of 32 degrees C, inoculum size of 5% and culturing time for 96 h the bioconversion ratio of GL into GA could reach 95%. A variety of parameters of submerged state fermentation, including the growth characteristics of A. parasiticus Speare BGB, the change amount of GL and GA, and the activity of beta-glucuronidase, were monitored simultaneously. GA was separated and purified by macroporous resin, silica gel column chromatography followed by recrystalization with the final purity over 98%. Purified product was identified as GA by the infrared absorption spectrum, molecular weight, and nuclear magnetic resonance. This study provided a new and efficient approach of obtaining GA by microbial transformation.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Aspergillus
/
Ácido Glicirrínico
/
Glycyrrhiza uralensis
/
Ácido Glicirretínico
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Prikl Biokhim Mikrobiol
Año:
2010
Tipo del documento:
Article