CYP450 biosensors based on screen-printed carbon electrodes for the determination of cocaine.
Anal Chim Acta
; 685(1): 15-20, 2011 Jan 24.
Article
en En
| MEDLINE
| ID: mdl-21168546
ABSTRACT
A new electrochemical method has been described and characterized for the determination of cocaine using screen-printed biosensors. The enzyme cytochrome P450 was covalently attached to screen-printed carbon electrodes. Experimental design methodology has been performed to optimize the pH and the applied potential, both variables that have an influence on the chronoamperometric determination of the drug. This method showed a reproducibility of 3.56% (n=4) related to the slopes of the calibration curves performed in the range from 19 up to 166nM. It has been probed the used of this kind of biosensors in the determination of cocaine in street samples, with an average capability of detection of 23.05±3.53nM (n=3, α=ß=0.05).
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Hidrocarburo de Aril Hidroxilasas
/
Técnicas Biosensibles
/
Cocaína Crack
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Anal Chim Acta
Año:
2011
Tipo del documento:
Article