PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1.
FEBS Lett
; 585(7): 1089-96, 2011 Apr 06.
Article
en En
| MEDLINE
| ID: mdl-21402070
ABSTRACT
LIMD1 is a tumour suppressor gene (TSG) down regulated in â¼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically, LIMD1 interacts with pRB, repressing E2F-driven transcription as well as being a critical component of microRNA-mediated gene silencing. In this study we show a CpG island within the LIMD1 promoter contains a conserved binding motif for the transcription factor PU.1. Mutation of the PU.1 consensus reduced promoter driven transcription by 90%. ChIP and EMSA analysis demonstrated that PU.1 specifically binds to the LIMD1 promoter. siRNA depletion of PU.1 significantly reduced endogenous LIMD1 expression, demonstrating that PU.1 is a major transcriptional activator of LIMD1.
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Base de datos:
MEDLINE
Asunto principal:
Activación Transcripcional
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Transactivadores
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Genes Supresores de Tumor
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Proteínas Proto-Oncogénicas
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Péptidos y Proteínas de Señalización Intracelular
Idioma:
En
Revista:
FEBS Lett
Año:
2011
Tipo del documento:
Article