Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53.
Proc Natl Acad Sci U S A
; 87(16): 6166-70, 1990 Aug.
Article
en En
| MEDLINE
| ID: mdl-2143581
ABSTRACT
To investigate the effect that human wild-type p53 (wt-p53) expression has on cell proliferation we constructed a recombinant plasmid, pM47, in which wt-p53 cDNA is under transcriptional control of the hormone-inducible mouse mammary tumor virus promoter linked to the dominant biochemical selection marker gene Eco gpt. The pM47 plasmid was introduced into T98G cells derived from a human glioblastoma multiforme tumor, and a stable clonal cell line, GM47.23, was derived that conditionally expressed wt-p53 following exposure to dexamethasone. We show that induction of wt-p53 expression in exponentially growing cells inhibits cell cycle progression and that the inhibitory effect is reversible upon removal of the inducer or infection with simian virus 40. Moreover, when growth-arrested cells are stimulated to proliferate, induction of wt-p53 expression inhibits G0/G1 progression into S phase and the cells accumulate with a DNA content equivalent to cells arrested in the G0/G1 phase of the cell cycle. Taken together, these studies suggest that wt-p53 may play a negative role in growth regulation.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Células Tumorales Cultivadas
/
Proteínas Oncogénicas
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1990
Tipo del documento:
Article