High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies.
Mol Ecol Resour
; 10(1): 193-6, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-21565009
ABSTRACT
High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.
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MEDLINE
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mol Ecol Resour
Año:
2010
Tipo del documento:
Article