2-DE and MALDI-TOF-MS analysis of therapeutic fusion protein abatacept.
Electrophoresis
; 32(12): 1438-43, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-21626521
ABSTRACT
2-DE and MALDI-TOF MS are useful techniques for the quality evaluation of medicinal products derived from recombinant DNA technology. The principal objective of this study has been to evaluate the suitability of 2-DE in combination with MALDI-TOF MS for the quality study of the therapeutic recombinant protein, abatacept. 1-DE SDS-PAGE, under reducing and nonreducing conditions, and 2-DE analysis were used for the assessment of M(r) , pI, and enzymatic deglycosylation efficiency of abatacept. 2-DE allowed the assessment of product identity, purity, charge heterogeneity, isoform pattern, and post-translational modifications. Furthermore, optimization of the deglycosylation procedure, charge heterogeneity, and sample preparation for the subsequent MALDI-TOF MS analysis has been addressed. PMF analysis allowed rapid identity confirmation of abatacept.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes de Fusión
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Electroforesis en Gel Bidimensional
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Inmunoconjugados
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Idioma:
En
Revista:
Electrophoresis
Año:
2011
Tipo del documento:
Article