Cathelin-related antimicrobial peptide differentially regulates T- and B-cell function.
Eur J Immunol
; 41(10): 3006-16, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21773974
ABSTRACT
Mammalian antimicrobial peptides (AMPs) play an important role in host defense via direct antimicrobial activity as well as immune regulation. The mouse cathelin-related antimicrobial peptide (mCRAMP), produced from the mouse gene Camp, is the only mouse cathelicidin identified and the ortholog of the human gene encoding the peptide LL-37. This study tested the hypothesis that mouse B and T cells produce and respond to mCRAMP. We show that all mature mouse B-cell subsets, including follicular (FO), marginal zone (MZ), B1a, and B1b cells, as well as CD4(+) and CD8(+) T cells produce Camp mRNA and mCRAMP protein. Camp(-/-) B cells produced equivalent levels of IgM, IgG3, and IgG2c but less IgG1 and IgE, while Camp(-/-) CD4(+) T cells cultured in Th2-inducing conditions produced more IL-4-expressing cells when compared with WT cells, effects that were reversed upon addition of mCRAMP. In vivo, Camp(-/-) mice immunized with TNP-OVA absorbed in alum produced an enhanced TNP-specific IgG1 response when compared with WT mice. ELISpot analysis revealed increased numbers of TNP-specific IgG1-secreting splenic B cells and FACS analysis revealed increased CD4(+) T-cell IL-4 expression. Our results suggest that mCRAMP differentially regulates B- and T-cell function and implicate mCRAMP in the regulation of adaptive immune responses.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Linfocitos B
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Linfocitos T CD4-Positivos
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Subgrupos de Linfocitos B
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Linfocitos T CD8-positivos
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Catelicidinas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Eur J Immunol
Año:
2011
Tipo del documento:
Article