ent-Kaurane diterpenoids from Croton tonkinensis stimulate osteoblast differentiation.
J Nat Prod
; 74(12): 2526-31, 2011 Dec 27.
Article
en En
| MEDLINE
| ID: mdl-22085418
ABSTRACT
Four new ent-kaurane diterpenoids (1-4) were isolated from the leaves of Croton tonkinensis by bioactivity-guided fractionation using an in vitro osteoblast differentiation assay. Their structures were identified as ent-11ß-acetoxykaur-16-en-18-ol (1), ent-11α-hydroxy-18-acetoxykaur-16-ene (2), ent-14ß-hydroxy-18-acetoxykaur-16-ene (3), and ent-7α-hydroxy-18-acetoxykaur-16-ene (4). Compounds 1-4 significantly increased alkaline phosphatase activity and osteoblastic gene promoter activity. Compounds 1-3 also increased the levels of ALP and collagen type I alpha mRNA in C2C12 cells in a dose-dependent manner. These results suggest that ent-kaurane diterpenoids from C. tonkinensis have a direct stimulatory effect on osteoblast differentiation and may be potential therapeutic molecules against bone diseases such as osteoporosis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Croton
/
Diterpenos de Tipo Kaurano
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Nat Prod
Año:
2011
Tipo del documento:
Article