Ultrafast hetero-nuclear 2D J-resolved spectroscopy.

ABSTRACT

Ultrafast techniques enable the acquisition of 2D NMR spectra in a single scan. In this study, we propose a new ultrafast experiment designed to record hetero-nuclear (1)H-(13)C J-resolved spectra in a fraction of a second. The approach is based on continuous constant-time phase modulated spatial encoding followed by a J-resolved detection scheme. An optional isotopic filter is implemented to remove the signal arising from (1)H bound to (12)C. While the most evident application of the technique proposed in this paper is the direct measurement of one bond scalar (13)C-(1)H couplings for structural elucidation purposes, it also offers interesting potentialities for measuring (13)C isotopic enrichments in metabolic samples. The main features of this methodology are presented, and the analytical performances of the ultrafast hetero-nuclear J-resolved pulse sequence are evaluated on model samples.