The mast cell stabilizer ketotifen reduces joint capsule fibrosis in a rabbit model of post-traumatic joint contractures.
Inflamm Res
; 61(4): 285-92, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22173279
ABSTRACT
OBJECTIVES:
Using a rabbit model of post-traumatic joint contractures, we investigated whether treatment with a mast cell stabilizer after joint injury would lessen the molecular manifestations of joint capsule fibrosis.METHODS:
Surgical joint injury was used to create stable post-traumatic contractures of the knee in skeletally mature New Zealand white rabbits. Four groups of animals were studied a non-operated control group (n = 8), an operated contracture group (n = 13) and two operated groups treated with the mast cell stabilizer, ketotifen, at doses of 0.5 mg/kg (n = 9) and 1.0 mg/kg (n = 9) twice daily. Joint capsule fibrosis was assessed by quantifying the mRNA and protein levels of α-SMA, tryptase, TGF-ß1, collagen I and collagen III. Significance was tested using an ANOVA analysis of variance.RESULTS:
The protein and mRNA levels of α-SMA, TGF-ß1, tryptase and collagen I and III were significantly elevated in the operated contracture group compared to control (p < 0.01). In both ketotifen-treated groups, protein and mRNA levels of α-SMA, TGF-ß1 and collagen I were significantly reduced compared to the operated contracture group (p < 0.01).CONCLUSIONS:
These data suggest an inflammatory pathway mediated by mast cell activation is involved in joint capsule fibrosis after traumatic injury.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Fibrosis
/
Antialérgicos
/
Contractura
/
Antagonistas de los Receptores Histamínicos H1
/
Cetotifen
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Inflamm Res
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Año:
2012
Tipo del documento:
Article