The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles.
J Gen Virol
; 93(Pt 10): 2290-2298, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22815270
ABSTRACT
Gene 3 in the genomes of several plant-infecting rhabdoviruses, including rice transitory yellowing virus (RTYV), has been postulated to encode a cell-to-cell movement protein (MP). Trans-complementation experiments using a movement-defective tomato mosaic virus and the P3 protein of RTYV, encoded by gene 3, facilitated intercellular transport of the mutant virus. In transient-expression experiments with the GFP-fused P3 protein in epidermal leaf cells of Nicotiana benthamiana, the P3 protein was associated with the nucleus and plasmodesmata. Immunogold-labelling studies of thin sections of RTYV-infected rice plants using an antiserum against Escherichia coli-expressed His(6)-tagged P3 protein indicated that the P3 protein was located in cell walls and on virus particles. In Western blots using antisera against E. coli-expressed P3 protein and purified RTYV, the P3 protein was detected in purified RTYV, whilst antiserum against purified RTYV reacted with the E. coli-expressed P3 protein. After immunogold labelling of crude sap from RTYV-infected rice leaves, the P3 protein, as well as the N protein, was detected on the ribonucleocapsid core that emerged from partially disrupted virus particles. These results provide evidence that the P3 protein of RTYV, which functions as a viral MP, is a viral structural protein and seems to be associated with the ribonucleocapsid core of virus particles.
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1
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
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Rhabdoviridae
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Oryza
/
Virión
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Proteínas de Movimiento Viral en Plantas
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Gen Virol
Año:
2012
Tipo del documento:
Article