Monitoring of methylergometrine in human breast milk by solid-phase extraction and high-performance liquid chromatography with fluorimetric detection.
Pharmazie
; 67(6): 482-4, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-22822533
ABSTRACT
A high-performance liquid chromatographic assay has been developed for the detection and quantification of the conventional postnatal uterotonic drug, methylergometrine, in human breast milk using a C-18 reversed-phase column by isocratic elution. The analytical method consisted of sample clean-up by solid-phase extraction, and the fluorescence detection required only 8.5 min per sample for separation and quantitation. This assay gave intra- and inter-assay coefficients of variation of less than 7.9% and 7.7%, respectively, and the detection limit was approximately 50 pg/ml. This method was applied for drug level monitoring in the breast milk of patients given methylergometrine.
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Base de datos:
MEDLINE
Asunto principal:
Oxitócicos
/
Metilergonovina
/
Leche Humana
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Pharmazie
Asunto de la revista:
FARMACIA
Año:
2012
Tipo del documento:
Article