Effect of methionine on production of naphthoquinones in Impatiens balsamina root cultures and detection of some secondary metabolites.
Pharm Biol
; 51(1): 36-41, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-22979941
ABSTRACT
CONTEXT Lawsone, lawsone methyl ether and 3,3'-methylelnebislawsone are the main active compounds of Impatiens balsamina L. (Balsaminaceae). These compounds possess various pharmacological activities that have been shown to assist with the treatment of skin diseases. OBJECTIVE:
This work focused on increased naphthoquinone production in I. basamina root cultures using methionine feeding. MATERIALS ANDMETHODS:
I. balsamina root cultures were maintained in liquid Gamborg's B5 medium supplemented with 0.1 mg/L α-naphthalene acetic acid, 0.1 mg/L kinetin, 1.0 mg/L 6-benzyladenine and 20 g/L sucrose. The effect of methionine concentration (50, 100, 300, 500 and 1000 mg/L) on naphthoquinone production of I. basamina root cultures was determined. Isolation of secondary metabolites from I. balsamina root cultures was also carried out. RESULTS ANDDISCUSSION:
Feeding of 300 mg/L methionine to the root cultures at the beginning of the growth cycle increased the production of 3,3'-methylelnebislawsone almost two-fold (0.63 mg/g dry weight, compared to the control group 0.32 mg/g dry weight). Optimization of the feeding conditions showed that adding 500 mg/L methionine to a 21-day old root cultures increased production of lawsone methyl ether and 3,3'-methylenebislawsone up to 2.6- and 3.1-fold higher, respectively, compared to the controls. In addition, various pharmacologically interesting secondary metabolites were isolated from I. balsamina root cultures, such as a flavonoid, luteolin, a naphthoquinone, 2,3-dihydroxy-1,4-naphthoquinone, and a triterpenoid, echinocystic acid. This is the first report of the occurrence of these compounds in this plant.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Naftoquinonas
/
Impatiens
/
Metionina
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Pharm Biol
Año:
2013
Tipo del documento:
Article