Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
Euro Surveill
; 17(39)2012 Sep 27.
Article
en En
| MEDLINE
| ID: mdl-23041020
ABSTRACT
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI) 2.56.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
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Base de datos:
MEDLINE
Asunto principal:
Infecciones por Coronavirus
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Euro Surveill
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2012
Tipo del documento:
Article