The Francisella tularensis LVS ΔpdpC mutant exhibits a unique phenotype during intracellular infection.
BMC Microbiol
; 13: 20, 2013 Jan 29.
Article
en En
| MEDLINE
| ID: mdl-23356941
ABSTRACT
BACKGROUND:
A prerequisite for the virulence of the facultative intracellular bacterium Francisella tularensis is effective intramacrophage proliferation, which is preceded by phagosomal escape into the cytosol, and ultimately leads to host cell death. Many components essential for the intracellular life cycle are encoded by a gene cluster, the Francisella pathogenicity island (FPI), constituting a type VI secretion system.RESULTS:
We characterized the FPI mutant ΔpdpC of the live vaccine strain (LVS) of F. tularensis and found that it exhibited lack of intracellular replication, incomplete phagosomal escape, and marked attenuation in the mouse model, however, unlike a phagosomally contained FPI mutant, it triggered secretion of IL-1ß, albeit lower than LVS, and markedly induced LDH release.CONCLUSIONS:
The phenotype of the ΔpdpC mutant appears to be unique compared to previously described F. tularensis FPI mutants.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Factores de Virulencia
/
Francisella tularensis
/
Macrófagos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
BMC Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2013
Tipo del documento:
Article