Inheritance of the henny feathering trait in the golden Campine chicken: evidence for allelism with the gene that causes henny feathering in the Sebright bantam.

ABSTRACT

The henny feathering mutation causes roosters to develop female feathering morphology as a result of increased conversion of androgen to estrogen (aromatase activity) in extraglandular tissues, including skin. This trait is maintained in two breeds of chickens the Sebright Bantam and the Golden Campine. To characterize the inheritance of this trait further, we did breeding studies of the Golden Campine and identified the trait by measuring aromatase activity in biopsied skin. As previously established for the Sebright Bantam, the trait is transmitted in the Campine by an autosomal, incomplete dominant mechanism; heterozygous offspring express half the levels of extraglandular aromatase as do homozygous Campines on average. No reversions to wild-type levels were observed in 555 heterozygous offspring of crosses between homozygous Campines and normals. Compound heterozygotes for the trait were obtained by mating homozygous Sebrights and homozygous Campines. When these compound heterozygote birds were crossed to control birds, all 98 offspring had elevated aromatase activity in skin, suggesting that the traits in Sebright and Campine birds are allelic. Furthermore, the restriction fragment length polymorphism pattern performed on genomic DNA was the same in the Sebright and Campine birds. Thus, the phenotypic, endocrine, and genetic features suggest that the traits in Sebright and Campine birds are the same. The trait in the Campine probably was derived from the Sebright.