Screen-printed biosensor based on the inhibition of the acetylcholinesterase activity for the determination of codeine.
Talanta
; 111: 8-12, 2013 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-23622519
ABSTRACT
The current paper presents the chronoamperometric determination of codeine using screen-printed carbon electrodes that incorporate tetrathiafulvalene in the matrix of the working electrode, as mediator, and cross-linked acetylcholinesterase. Applying a potential of +250 mV, a 1mM solution of acetylthiocholine in electrolyte solution pH 7 gives an oxidation signal due to the dimerization of its metabolite after the reaction with the enzyme. This electrochemical signal is decreased by consecutive additions of a solution of codeine, which allows the performance of curves of calibration for the validation of this electrochemical method, giving a reproducibility of 3.31% (n=6) and a capability of detection of 20 µM. This type of inhibition has been studied by means of a Lineweaver-Burk plot. Additionally, the developed biosensor was used for the determination of the quantity of codeine in pharmaceutical commercial tablets and urine samples.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Acetilcolinesterasa
/
Técnicas Biosensibles
/
Codeína
/
Técnicas Electroquímicas
Idioma:
En
Revista:
Talanta
Año:
2013
Tipo del documento:
Article