A novel approach to capillary plasma microsampling for quantitative bioanalysis.
Bioanalysis
; 5(9): 1131-5, 2013 May.
Article
en En
| MEDLINE
| ID: mdl-23641701
ABSTRACT
BACKGROUND:
A novel device and procedure for the collection and isolation of microvolumes of plasma have been developed and two pilot rodent PK studies have been completed.RESULTS:
This method involves collection of blood into a plastic-wrapped, EDTA-coated capillary tube, containing a small amount of a thixotropic gel and a porous plug. Following blood collection, the capillary is placed into a secondary labeled container suitable for centrifugation and plasma is generated. During centrifugation, the thixotropic gel isolates the plasma from the red blood cells and creates a physical barrier between the two matrices. The plasma is then dispensed from the capillary tube into a separate container for storage or processing.CONCLUSION:
A simple and robust novel approach for the collection of small plasma volumes from rodent TK studies has been demonstrated.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Bioensayo
/
Recolección de Muestras de Sangre
Idioma:
En
Revista:
Bioanalysis
Año:
2013
Tipo del documento:
Article