Analysis of microRNA and protein transfer by exosomes during an immune synapse.
Methods Mol Biol
; 1024: 41-51, 2013.
Article
en En
| MEDLINE
| ID: mdl-23719941
ABSTRACT
Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has shown that this transfer is enhanced by the formation of a functional immune synapse. Here we give a detailed description of the isolation of exosomes produced by immune cells by ultracentrifugation, their quantification by flow cytometry, and the analysis of miRNA and protein exchange between T cells and APCs, both at a distance and after the formation of an immune synapse.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Células Dendríticas
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Linfocitos B
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Linfocitos T
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MicroARNs
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Sinapsis Inmunológicas
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Exosomas
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Tetraspanina 30
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2013
Tipo del documento:
Article