High-level production of calcium malate from glucose by Penicillium sclerotiorum K302.
Bioresour Technol
; 143: 674-7, 2013 Sep.
Article
en En
| MEDLINE
| ID: mdl-23831380
ABSTRACT
In this study, after screening of 9 fungal strains for their ability to produce calcium malate, it was found that Penicillium sclerotiorum K302 among them could produce high-level of calcium malate. Under the optimal conditions, the titer of calcium malate in the supernatant was 88.6 g/l at flask level. During 10-l fermentation, the titer of 92.0 g/l, the yield of 0.88 g/g of glucose and the productivity of 1.23 g/l/h were reached within 72 h of the fermentation, demonstrating that the titer, yield and productivity of calcium malate by this strain were very high and the fermentation period was very short. After analysis of the partially purified product with HPLC, it was found that the main product was calcium malate. The results showed that P. sclerotiorum K302 obtained in this study was suitable for developing a novel one-step fermentation process for calcium malate production from glucose on large scale.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Penicillium
/
Calcio
/
Glucosa
/
Malatos
Idioma:
En
Revista:
Bioresour Technol
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2013
Tipo del documento:
Article