Locus-specific proteomics by TChP: targeted chromatin purification.
Cell Rep
; 4(3): 589-600, 2013 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-23911284
ABSTRACT
Here, we show that transcription factors bound to regulatory sequences can be identified by purifying these unique sequences directly from mammalian cells in vivo. Using targeted chromatin purification (TChP), a double-pull-down strategy with a tetracycline-sensitive "hook" bound to a specific promoter, we identify transcription factors bound to the repressed γ-globin gene-associated regulatory regions. After validation of the binding, we show that, in human primary erythroid cells, knockdown of a number of these transcription factors induces γ-globin gene expression. Reactivation of γ-globin gene expression ameliorates the symptoms of ß-thalassemia and sickle cell disease, and these factors provide potential targets for the development of therapeutics for treating these patients.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cromatina
/
Técnicas de Silenciamiento del Gen
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Cell Rep
Año:
2013
Tipo del documento:
Article