Rapid method of measuring the inhibition of calcium-oxalate monohydrate growth in urine.

ABSTRACT

In order to have a rapid method of measuring the inhibition of calcium-oxalate monohydrate growth in freshly voided whole urine, a test system by Meyer and Smith that has originally been developed for diluted urine was modified. The crystallization processes were monitored by an ion-selective calcium electrode, which allowed determination of the half-life value of the decrease in calcium within 25 min. Even given the high inhibitory activity of whole urine, the test gave reliable results when a high seed concentration was used. Inhibition was expressed as the ratio between the half-lives of the calcium decrease obtained in the presence and in the absence of inhibitors. This approach allowed kinetic studies of individual inhibitors in model solutions. Furthermore, the measurements of inhibitors in urine could be performed before the chemical composition was determined.