Mosaicism of HTLV-1 5' LTR CpG methylation in the absence of malignancy.
Virus Res
; 178(2): 452-61, 2013 Dec 26.
Article
en En
| MEDLINE
| ID: mdl-24036230
ABSTRACT
Viral expression varies widely between untransformed HTLV-1 positive clones derived from infected individuals without malignancy. Here we show that, in the absence of malignancy, 68% of HTLV-1 positive clones carry deleted (10%) or methylated (58%) 5' LTR. These changes were found to contribute to the fluctuation of viral expression between clones. 5' LTR deletions strongly impaired tax expression and thereby clonal expansion, telomere homeostasis, and genetic instability. The effects of CpG methylation on viral transcription were qualitatively and quantitatively different from those of LTR deletions and preserved the preleukemic features of HTLV-1(+)CD4(+) clones. 5' LTR methylation varied not only between clones but also between cells belonging to the same clones, between distinct integrated sequences within the same cells, and between CpG dyads along the 5' LTR. Such distributions suggest that a dynamic methylation spectrum might help sustain persistent infection.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Virus Linfotrópico T Tipo 1 Humano
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Infecciones por HTLV-I
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Metilación de ADN
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Secuencias Repetidas Terminales
Idioma:
En
Revista:
Virus Res
Asunto de la revista:
VIROLOGIA
Año:
2013
Tipo del documento:
Article