Intracellular mobility and nuclear trafficking of the stress-activated kinase JNK1 are impeded by hyperosmotic stress.

Misheva, Mariya; Kaur, Gurpreet; Ngoei, Kevin R W; Yeap, Yvonne Y; Ng, Ivan H W; Wagstaff, Kylie M; Ng, Dominic C H; Jans, David A; Bogoyevitch, Marie A

Misheva, Mariya; Kaur, Gurpreet; Ngoei, Kevin R W; Yeap, Yvonne Y; Ng, Ivan H W; Wagstaff, Kylie M; Ng, Dominic C H; Jans, David A; Bogoyevitch, Marie A.

Afiliación

Misheva M; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia.
Kaur G; Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia; European Molecular Biology Laboratory (EMBL) Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia.
Ngoei KR; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia.
Yeap YY; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia.
Ng IH; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia; Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia.
Wagstaff KM; Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia.
Ng DC; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia.
Jans DA; Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia.
Bogoyevitch MA; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Victoria 3010, Australia. Electronic address: marieb@unimelb.edu.au.

Biochim Biophys Acta ; 1843(2): 253-64, 2014 Feb.

Article en En | MEDLINE | ID: mdl-24184208

Asunto(s)

Núcleo Celular/metabolismo; Espacio Intracelular/metabolismo; Proteína Quinasa 8 Activada por Mitógenos/metabolismo; Presión Osmótica; Sorbitol/farmacología; Estrés Fisiológico; Animales; Antígenos Transformadores de Poliomavirus/metabolismo; Arsenitos/farmacología; Núcleo Celular/efectos de los fármacos; Activación Enzimática/efectos de los fármacos; Recuperación de Fluorescencia tras Fotoblanqueo; Proteínas Fluorescentes Verdes/metabolismo; Células HeLa; Humanos; Espacio Intracelular/efectos de los fármacos; Carioferinas/metabolismo; Cinética; Ratones; Presión Osmótica/efectos de los fármacos; Fosforilación/efectos de los fármacos; Transporte de Proteínas/efectos de los fármacos; Ratas; Estrés Fisiológico/efectos de los fármacos; Fracciones Subcelulares/enzimología

Palabras clave

5-iodoacetamidofluorescein; CLSM; ERK; FRAP; Fc; Fluorescence recovery after photobleaching; Fn; Fn/c; GFP; GST; HTC cells; IAF; Imp; JNK; Live-imaging; MAPK; MAPK kinase; MEFs; MKK; NLS; NTS; Nuclear import; SEM; Simian Virus SV40 large tumor antigen; Sorbitol; TAg; TR70; Texas Red-labeled dextran; c-Jun N-terminal kinase; confocal laser scanning microscopy; cytoplasmic fluorescence; extracellular signal-regulated kinase; fluorescence recovery after photobleaching; glutathione S-transferase; green fluorescent protein; importin; mitogen-activated protein kinase; murine embryonic fibroblasts; nuclear factor erythroid 2 (NF-E2); nuclear fluorescence; nuclear localization sequence; nuclear translocation signal; p45; rat hepatoma tissue culture cells; standard error of the mean; t(1/2); the nuclear to cytoplasmic ratio; time to reach half-maximal recovery

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Presión Osmótica / Sorbitol / Estrés Fisiológico / Núcleo Celular / Espacio Intracelular / Proteína Quinasa 8 Activada por Mitógenos Idioma: En Revista: Biochim Biophys Acta Año: 2014 Tipo del documento: Article

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