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Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B.
Lin, B-W; Wang, Y-C; Chang-Liao, P-Y; Lin, Y-J; Yang, S-T; Tsou, J-H; Chang, K-C; Liu, Y-W; Tseng, J T; Lee, C-T; Lee, J-C; Hung, L-Y.
Afiliación
  • Lin BW; Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Wang YC; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
  • Chang-Liao PY; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
  • Lin YJ; Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Yang ST; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
  • Tsou JH; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
  • Chang KC; Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Liu YW; Department of Pathology, Kuo General Hospital, Tainan, Taiwan.
  • Tseng JT; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
  • Lee CT; Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Lee JC; Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
  • Hung LY; 1] Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan [2] Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan [3] Infectious Disease and Signaling Research Center,
Cell Death Dis ; 5: e1106, 2014 Mar 06.
Article en En | MEDLINE | ID: mdl-24603334
ABSTRACT
The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Puntos de Control de la Fase M del Ciclo Celular / Aurora Quinasa B / Aurora Quinasa C / Huso Acromático Idioma: En Revista: Cell Death Dis Año: 2014 Tipo del documento: Article
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Puntos de Control de la Fase M del Ciclo Celular / Aurora Quinasa B / Aurora Quinasa C / Huso Acromático Idioma: En Revista: Cell Death Dis Año: 2014 Tipo del documento: Article