Identification of Acinetobacter clinical isolates by polymerase chain reaction analysis of 16S-23S ribosomal ribonucleic acid internal transcribed spacer.
Indian J Med Microbiol
; 32(2): 143-7, 2014.
Article
en En
| MEDLINE
| ID: mdl-24713899
ABSTRACT
BACKGROUND:
The genus Acinetobacter is a diverse group of Gram-negative bacteria involve at least 33 species using the molecular methods. Although the genus Acinetobacter comprises a number of definite bacterial species, some of these species are of clinical importance. Therefore, it is of vital importance to use a method which is able to reliably and efficiently differentiate the numerous Acinetobacter species.OBJECTIVES:
This study aims to identify Acinetobacter of clinical isolates from Assir region to the species level by 16S-23S intergenic spacers internal transcribed spacer (ITS) of ribosomal ribonucleic acid (rRNA). MATERIALS ANDMETHODS:
Deoxyribonucleic acid extraction, polymerase chain reaction amplification of 16S-23S intergenic spacer sequences (ITS) was performed using the bacterium-specific universal primers.RESULTS:
Based on the 16S-23S intergenic spacers (ITS) of rRNA sequences, all isolates tested were identified as Acinetobacter baumannii. The isolates shared a common ancestral lineage with the prototypes A. baumannii U60279 and U60280 with 99% sequence similarities.CONCLUSION:
These findings confirmed 16S-23S rRNA ITS for the identification of A. baumannii of different genotypes among patients.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Acinetobacter
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ARN Ribosómico 16S
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ARN Ribosómico 23S
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Reacción en Cadena de la Polimerasa
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Técnicas de Tipificación Bacteriana
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ADN Espaciador Ribosómico
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Indian J Med Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2014
Tipo del documento:
Article