Human B cell lines secreting IgM antibody specific for myelin basic protein.
J Neuroimmunol
; 24(1-2): 9-16, 1989 Sep.
Article
en En
| MEDLINE
| ID: mdl-2478580
ABSTRACT
In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5-12 micrograms/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occurred with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.
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Base de datos:
MEDLINE
Asunto principal:
Inmunoglobulina M
/
Linfocitos B
/
Proteína Básica de Mielina
Idioma:
En
Revista:
J Neuroimmunol
Año:
1989
Tipo del documento:
Article