Type II PtdIns 4-kinase ß associates with CD4-p56lck complex and is involved in CD4 receptor signaling.
Mol Cell Biochem
; 395(1-2): 231-9, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-24972704
ABSTRACT
Type II phosphatidylinositol (PtdIns) 4-kinases are involved in the synthesis of PtdIns 4-phosphates and modulate various cell functions like, intracellular signaling, cytoskeletal rearrangements, vesicular trafficking, and pathogen invasion. In CD3 receptor activated T cells, a type II PtdIns 4-kinase ß is recruited to CD3 receptor zeta and plays a role in intracellular calcium release and probably in actin cytoskeleton reorganization. T cell receptor mediated activation is supported by CD4 receptor. The role of type II PtdIns 4-kinase ß in CD4 receptor-mediated signaling was addressed in the present manuscript. Crosslinking of CD4 receptors with monoclonal antibodies showed an increase in CD4-associated PtdIns 4-kinase activity and requires p56(lck) activity. Biochemical characterization suggests that it belongs to type II PtdIns 4-kinase family. shRNA mediated knockdown of type II PtdIns 4-kinase ß showed abrogation of CD4 receptor induced intracellular calcium release. These results suggest that type II PtdIns 4-kinase ß plays an integral part in CD4 receptor-mediated signaling.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Transducción de Señal
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Antígenos CD4
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Fosfotransferasas (Aceptor de Grupo Alcohol)
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Proteína Tirosina Quinasa p56(lck) Específica de Linfocito
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2014
Tipo del documento:
Article