Involvement of connexin43 in the acute hyperosmotic stimulusinduced synthesis and release of vasopressin in the supraoptic nucleus of rats.
Mol Med Rep
; 10(4): 2165-71, 2014 Oct.
Article
en En
| MEDLINE
| ID: mdl-25050982
ABSTRACT
There is evidence that astroglial connexin43 (Cx43) in the supraoptic nucleus (SON) is required for the hyperosmolarityinduced increase in Fos protein expression in magnocellular neurosecretory cells (MNCs). In the present study, the role of astroglial Cx43 in the synthesis and release of vasopressin (VP) by MNCs in the SON subjected to hyperosmotic stimulus was examined. The results revealed that the VP levels in the SON and plasma were increased following acute hyperosmotic stimulus. Treatment of MNCs with Cx43specific antisense oligodeoxynucleotides (ASODN), which temporarily reduced Cx43 protein production, limited the VP synthesis and release induced by a hyperosmotic stimulus. Similarly, the addition of gap junction and Cx43 hemichannel blockers also attenuated the VP synthesis and release induced by an acute hyperosmotic stimulus. A high extracellular [Ca2+]([Ca2+]o) has been demonstrated to reduce the gap junction activity or opening probability of Cx54 hemichannels. Notably, it was identified that high [Ca2+]o attenuated the VP synthesis and release induced by acute hyperosmotic stimulus, while low [Ca2+]o had a weak or no effect. These results suggested that Cx43 participates in the VP synthesis and release induced by hyperosmotic stimulation in the SON.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Presión Osmótica
/
Núcleo Supraóptico
/
Vasopresinas
/
Conexina 43
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Med Rep
Año:
2014
Tipo del documento:
Article