The effect of chicken extract on ERK/CREB signaling is ApoE isoform-dependent.
Food Funct
; 5(9): 2043-51, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25080220
ABSTRACT
It is unclear how the nutritional supplement chicken extract (CE) enhances cognition. Human apolipoprotein E (ApoE) can regulate cognition and this isoform-dependent effect is associated with the N-methyl-d-aspartate receptor (NMDAR). To understand if CE utilizes this pathway, we compared the NMDAR signaling in neuronal cells expressing ApoE3 and ApoE4. We observed that CE increased S896 phosphorylation on NR1 in ApoE3 cells and this was linked to higher protein kinase C (PKC) activation. However, ApoE4 cells treated with CE have lowered S897 phosphorylation on NR1 and this was associated with reduced protein kinase A (PKA) phosphorylation. In ApoE3 cells, CE increased calmodulin kinase II (CaMKII) activation and AMPA GluR1 phosphorylation on S831. In contrast, CE reduced CaMKII phosphorylation and led to higher de-phosphorylation of S831 and S845 on GluR1 in ApoE4 cells. While CE enhanced ERK/CREB phosphorylation in ApoE3 cells, this pathway was down-regulated in both ApoE4 and mock cells after CE treatment. These results show that CE triggers ApoE isoform-specific changes on ERK/CREB signaling.
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1
Base de datos:
MEDLINE
Asunto principal:
Productos Avícolas
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Transducción de Señal
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Proteína de Unión a Elemento de Respuesta al AMP Cíclico
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Cognición
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Quinasas MAP Reguladas por Señal Extracelular
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Apolipoproteína E3
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Apolipoproteína E4
Idioma:
En
Revista:
Food Funct
Año:
2014
Tipo del documento:
Article