A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model.
J Infect Dis
; 211(5): 791-800, 2015 Mar 01.
Article
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| MEDLINE
| ID: mdl-25246535
ABSTRACT
Since the first reports in early 2013, >440 human cases of infection with avian influenza A(H7N9) have been reported including 122 fatalities. After the isolation of the first A(H7N9) viruses, the nucleotide sequences became publically available. Based on the coding sequence of the influenza virus A/Shanghai/2/2013 hemagglutinin gene, a codon-optimized gene was synthesized and cloned into a recombinant modified vaccinia virus Ankara (MVA). This MVA-H7-Sh2 viral vector was used to immunize ferrets and proved to be immunogenic, even after a single immunization. Subsequently, ferrets were challenged with influenza virus A/Anhui/1/2013 via the intratracheal route. Unprotected animals that were mock vaccinated or received empty vector developed interstitial pneumonia characterized by a marked alveolitis, accompanied by loss of appetite, weight loss, and heavy breathing. In contrast, animals vaccinated with MVA-H7-Sh2 were protected from severe disease.
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Base de datos:
MEDLINE
Asunto principal:
Virus Vaccinia
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Portadores de Fármacos
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Vacunas contra la Influenza
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Infecciones por Orthomyxoviridae
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Glicoproteínas Hemaglutininas del Virus de la Influenza
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Subtipo H7N9 del Virus de la Influenza A
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Vectores Genéticos
Idioma:
En
Revista:
J Infect Dis
Año:
2015
Tipo del documento:
Article