Your browser doesn't support javascript.
loading
[Protective effect of rosmarinic acid on hypoxia/reoxygenation injury in cardiomyocytes].
Zhongguo Zhong Yao Za Zhi ; 39(10): 1897-901, 2014 May.
Article en Zh | MEDLINE | ID: mdl-25282902
ABSTRACT

OBJECTIVE:

To study the protective effect of rosmarinic acid (Ros A) on the primary cardiomyocyte hypoxia/reoxygenation (H/R) injury.

METHOD:

Primary cardiomyocytes of rats were cultured in vitro to establish the H/R injury of cardiomyocytes and observe the changes in the cell viability and LDH leakage. The changes in ATP content and ROS in cardiomyocytes were measured by using chemiluminescence and fluorescent probe technique. The effects of rosmarinic acid on the apoptosis of cardiomyocytes, cleaved-caspase 3, Akt and p-Akt protein expression were further detected by flow cytometry and western blot analysis.

RESULT:

According to the experimental results, Ros A at doses of 25, 50, 100 mg x L(-1) could inhibit the decrease in H/R-induced cell viability, LDH leakage and excessive ROS generation, and maintain the ATP level in cells. Ros A at doses of 50, 100 mg x L(-1) could remarkably inhibit the H/R-induced cardiomyocyte apoptosis and down-regulate the expression of cleaved caspase-3. Moreover, Ros A at doses of 100 mg x L(-1) could significantly up-regulate the expression of p-Akt.

CONCLUSION:

Ros A has the significant effect in resisting the cardiomyocyte H/R injury, improve cardiomyocyte energy metabolism and reduce cell apoptosis, which is related to the activation of Akt pathway.
Asunto(s)
Buscar en Google
Base de datos: MEDLINE Asunto principal: Oxígeno / Extractos Vegetales / Cinamatos / Sustancias Protectoras / Rosmarinus / Miocitos Cardíacos / Depsidos / Hipoxia Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2014 Tipo del documento: Article
Buscar en Google
Base de datos: MEDLINE Asunto principal: Oxígeno / Extractos Vegetales / Cinamatos / Sustancias Protectoras / Rosmarinus / Miocitos Cardíacos / Depsidos / Hipoxia Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2014 Tipo del documento: Article