Antiherpetic mechanism of a sulfated derivative of Agaricus brasiliensis fruiting bodies polysaccharide.
Intervirology
; 57(6): 375-83, 2014.
Article
en En
| MEDLINE
| ID: mdl-25359160
ABSTRACT
OBJECTIVE:
To study the anti-herpes simplex virus (HSV) activity of a (1â6)-(1â3)-ß-D-glucan isolated from Agaricus brasiliensis fruiting bodies (FR) as well as its chemically sulfated derivative (FR-S).METHODS:
The antiherpetic activity and mechanism of action was studied by viral plaque assay applying different methodological strategies.RESULTS:
Although FR presented no in vitro antiherpetic action at 1 mg/ml, FR-S displayed promising anti-HSV-1 and anti-HSV-2 activities in both simultaneous and postinfection treatments, resulting in selectivity indices (CC50/EC50) higher than 393. FR-S had no virucidal effect, but significantly suppressed HSV-1 (EC50 = 0.32 µg/ml) and HSV-2 (EC50 = 0.10 µg/ml) adsorption. FR-S was less effective on adsorption inhibition of mutant virus strains devoid of gC (HSV-1 gCâ»39 and HSV-2 gCneg1), indicating a possible interaction with this glycoprotein. The reduction of viral adsorption upon cell pretreatment with FR-S also suggests its interaction with cellular components. FR-S inhibited HSV-1 (EC50 = 8.39 µg/ml) and HSV-2 (EC50 = 2.86 µg/ml) penetration more efficiently than heparin. FR-S reduced HSV-1 and HSV-2 cell-to-cell spread. A synergic effect between FR-S and acyclovir was also detected.CONCLUSIONS:
FR-S displays an interesting mechanism of antiviral action and represents a promising candidate for the treatment and/or prevention of herpetic infections, to be used as a single therapeutic agent or in combination with acyclovir.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Antivirales
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Polisacáridos
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Agaricus
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Herpesvirus Humano 2
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Herpesvirus Humano 1
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Cuerpos Fructíferos de los Hongos
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Beta-Glucanos
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Intervirology
Año:
2014
Tipo del documento:
Article