Andrographolide interferes quorum sensing to reduce cell damage caused by avian pathogenic Escherichia coli.
Vet Microbiol
; 174(3-4): 496-503, 2014 Dec 05.
Article
en En
| MEDLINE
| ID: mdl-25448450
ABSTRACT
Avian pathogenic Escherichia coli (APEC) induce septicemia in chickens by invading type II pneumocytes to breach the blood-air barrier. The virulence of APEC can be regulated by quorum sensing (QS). Andrographolide is a QS inhibitor of Pseudomonas aeruginosa (P. aeruginosa). Therefore, we investigate whether andrographolide inhibits the injury of chicken type II pneumocytes by avian pathogenic E. coli O78 (APEC-O78) by disrupting the bacterial QS system. The results showed that sub-MIC of andrographolide significantly reduced the release of lactate dehydrogenase (LDH), F-actin cytoskeleton polymerization, and the degree of the adherence to chicken type II pneumocytes induced by APEC-O78. Further, we found that andrographolide significantly decreased the autoinducer-2 (AI-2) activity and the expression of virulence factors of APEC-O78. These results suggest that andrographolide reduce the pathogenicity of APEC-O78 in chicken type II pneumocytes by interfering QS and decreasing virulence. These results provide new evidence for colibacillosis prevention methods in chickens.
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MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
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Pollos
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Diterpenos
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Escherichia coli
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Infecciones por Escherichia coli
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Percepción de Quorum
Idioma:
En
Revista:
Vet Microbiol
Año:
2014
Tipo del documento:
Article