Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins.
Anal Biochem
; 477: 95-7, 2015 May 15.
Article
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| MEDLINE
| ID: mdl-25454506
ABSTRACT
A diverse set of SUMO target proteins has been identified. Therefore, there is a growing interest in studying sumoylation and SUMO interactions in cells. When the sumoylation of a protein or a SUMO interaction is suspected, a standard co-immunoprecipitation analysis using anti-SUMO and anti-target protein antibody is usually performed as a first step. However, the identification of endogenous sumoylated proteins is challenging because of the activity of isopeptidases, and often only a small fraction of a target protein is sumoylated at a given time. Here, we briefly summarize several important steps to ensure a successful co-immunoprecipitation analysis to detect possible sumoylation.
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MEDLINE
Asunto principal:
Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina
/
Mapeo de Interacción de Proteínas
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Sumoilación
Idioma:
En
Revista:
Anal Biochem
Año:
2015
Tipo del documento:
Article