In vivo ultrasound assessment of gastric emptying in newborn mice.
J Pediatr Gastroenterol Nutr
; 60(3): 322-6, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25714576
ABSTRACT
OBJECTIVES:
The aim of the present study was to develop an ultrasonographic approach to comparatively assess gastric emptying in newborn wild-type and guanosine triphosphate cyclohydrolase knockout hph-1 mice, because we previously reported gastroparesis early in life in this strain.METHODS:
Stomach transverse, anteroposterior, and longitudinal ultrasonographic measurements were obtained with a 40-MHz transducer in pups immediately after maternal separation and 4 hours later. A conventional equation was used and the predicted values validated by obtaining postmortem gastric content volume measurements. Wild-type and hph-1 mice gastric emptying rates were comparatively evaluated at 1 to 3 and 5 to 8 days of age, respectively.RESULTS:
The ultrasound equation closely predicted the newborn stomach content volumes with a correlation coefficient (R) of 0.93 and 0.81 (P < 0.01) for measurements obtained on full stomach and after 4 hours of fasting, respectively. In wild-type mice, gastric emptying was age dependent and associated with a greater residual volume at 1 to 3 days (65% ± 7%), as compared with 5- to 8-day-old pups (33% ± 4%; P â< 0.01), after fasting. In contrast, an equal duration of fasting resulted in a significantly greater residual gastric content volume in 5- to 8-day-old hph-1 mice (68%â ± 7%; P < 0.01), as compared with same-age wild-type mice.CONCLUSIONS:
Ultrasonography offers a sensitive and accurate estimate of gastric content volume in newborn mice. In wild-type newborn mice, gastric emptying rate is age dependent and significantly reduced in the immediate postnatal period. The newborn hph-1 mice have a significantly higher gastric residual volume, as compared with wild-type same-age animals.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Estómago
/
Gastroparesia
/
Vaciamiento Gástrico
/
Mucosa Gástrica
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
J Pediatr Gastroenterol Nutr
Año:
2015
Tipo del documento:
Article