Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.
Vet Res
; 46: 14, 2015 Feb 24.
Article
en En
| MEDLINE
| ID: mdl-25828907
ABSTRACT
Enterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
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Fimbrias Bacterianas
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Adhesinas de Escherichia coli
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Diarrea
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Infecciones por Escherichia coli
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Escherichia coli Enterotoxigénica
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Anticuerpos de Dominio Único
Idioma:
En
Revista:
Vet Res
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2015
Tipo del documento:
Article