Roary: rapid large-scale prokaryote pan genome analysis.
Bioinformatics
; 31(22): 3691-3, 2015 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-26198102
ABSTRACT
UNLABELLED A typical prokaryote population sequencing study can now consist of hundreds or thousands of isolates. Interrogating these datasets can provide detailed insights into the genetic structure of prokaryotic genomes. We introduce Roary, a tool that rapidly builds large-scale pan genomes, identifying the core and accessory genes. Roary makes construction of the pan genome of thousands of prokaryote samples possible on a standard desktop without compromising on the accuracy of results. Using a single CPU Roary can produce a pan genome consisting of 1000 isolates in 4.5 hours using 13 GB of RAM, with further speedups possible using multiple processors. AVAILABILITY AND IMPLEMENTATION Roary is implemented in Perl and is freely available under an open source GPLv3 license from http//sanger-pathogens.github.io/Roary CONTACT roary@sanger.ac.uk SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
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Base de datos:
MEDLINE
Asunto principal:
Células Procariotas
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Programas Informáticos
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Genoma Bacteriano
Idioma:
En
Revista:
Bioinformatics
Asunto de la revista:
INFORMATICA MEDICA
Año:
2015
Tipo del documento:
Article