In vitro neutralization of prions with PrP(Sc)-specific antibodies.
Prion
; 9(4): 292-303, 2015.
Article
en En
| MEDLINE
| ID: mdl-26284508
ABSTRACT
Prion diseases reflect the misfolding of a self-protein (PrP(C)) into an infectious, pathological isomer (PrP(Sc)). By targeting epitopes uniquely exposed by misfolding, our group developed PrP(Sc)-specific vaccines to 3 disease specific epitopes (DSEs). Here, antibodies induced by individual DSE vaccines are evaluated for their capacity to neutralize prions in vitro. For both purified antibodies and immunoreactive sera, the PrP(Sc)-specific antibodies were equally effective in neutralizing prions. Further, there was no significant increase in neutralizing activity when multiple DSEs were targeted within an assay. At a low antibody concentration, the PrP(Sc)-specific antibodies matched the neutralization achieved by an antibody that may act via both PrP(C) and PrP(Sc). At higher doses, however, this pan-specific antibody was more effective, potentially due to a combined deactivation of PrP(Sc) and depletion of PrP(C).
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Priones
/
Proteínas PrPSc
/
Anticuerpos
Idioma:
En
Revista:
Prion
Asunto de la revista:
BIOQUIMICA
Año:
2015
Tipo del documento:
Article