Interferon-stimulated gene factor 3 complex is required for the induction of sterile α motif and HD domain-containing protein 1 expression by interferon-α in SMMC-7721 cells.
Mol Med Rep
; 12(5): 7176-80, 2015 Nov.
Article
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| MEDLINE
| ID: mdl-26397446
ABSTRACT
Sterile α motif and HD domain-containing protein 1 (SAMHD1) is a novel intrinsic restriction factor that inhibits the replication of certain retroviruses and DNA viruses through its deoxynucleoside triphosphate triphosphohydrolase activity. A previous study by our group showed that SAMHD1 restrained hepatitis B virus replication and interferon (IFN)α induced SAMHD1 expression in liver cells. However the mechanisms of SAMHD1 upregulation by IFNα in liver cells have remained elusive. The present study demonstrated that IFNα treatment increased SAMHD1 mRNA levels in SMMC7721 cells in a timedependent manner. Knockdown of STAT1 inhibited the induction of SAMHD1 expression by IFNα in SMMC7721 cells. STAT2 silencing also suppressed the induction of SAMHD1 expression by IFNα in SMMC7721 cells. Furthermore, the induction of SAMHD1 expression in SMMC7721 cells by IFNα was found to be dependent on IFNregulatory factor 9 (IRF9). In conclusion, these results suggested that the interferonstimulated gene factor 3 complex, which consists of STAT1, STAT2 and IRF9, is required for the induction of SAMHD1 expression by IFN-α in SMMC-7721 cells.
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Base de datos:
MEDLINE
Asunto principal:
Interferón-alfa
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Proteínas de Unión al GTP Monoméricas
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Factor 3 de Genes Estimulados por el Interferón
Idioma:
En
Revista:
Mol Med Rep
Año:
2015
Tipo del documento:
Article