Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS.
Methods Mol Biol
; 1383: 29-37, 2016.
Article
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| MEDLINE
| ID: mdl-26660171
ABSTRACT
Antiepileptic drugs (AEDs) are a diverse group of pharmacological agents used in the treatment of epileptic seizures. Over the past several decades some new AEDs, including lamotrigine (LTG), levetiracetam (LVA), oxcarbazepine (OXC), topiramate (TOP), and zonisamide (ZNS), have become widely used. This chapter describes a very simple and rapid liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of LVA, ZNS, LTG, TOP, and MHD in human serum. The method requires a very small amount of serum (50 µL) for multiple drug measurements and has a total analysis time of 4 min that makes it well suited for routine clinical analysis of several drugs simultaneously. The imprecision (CVs) measured at various concentrations across the analytical measurement range (AMR) are less than 7% for all analytes. The AMR for each analyte is as follows LVA (1-100 µg/mL), ZNS (0.8-80 µg/mL), TOP (0.5-50 µg/mL), and 0.6-60 µg/mL for LTG and MHD.
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Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Piracetam
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Triazinas
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Carbamazepina
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Monitoreo de Drogas
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Fructosa
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Isoxazoles
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Anticonvulsivantes
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article