DNA-directed spatial assembly of photosynthetic light-harvesting proteins.
Org Biomol Chem
; 14(4): 1359-62, 2016 Jan 28.
Article
en En
| MEDLINE
| ID: mdl-26660647
ABSTRACT
This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Rhodopseudomonas
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ADN
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Complejos de Proteína Captadores de Luz
Idioma:
En
Revista:
Org Biomol Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2016
Tipo del documento:
Article