An AlphaScreen Assay for the Discovery of Synthetic Chemical Inhibitors of Glucagon Production.
J Biomol Screen
; 21(4): 325-32, 2016 Apr.
Article
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| MEDLINE
| ID: mdl-26676097
ABSTRACT
Glucose homeostasis is primarily controlled by two opposing hormones, insulin and glucagon, and diabetes results when insulin fails to inhibit glucagon action. Recent efforts to control glucagon in diabetes have focused on antagonizing the glucagon receptor, which is effective in lowering blood glucose levels but leads to hyperglucogonemia in rodents. An alternative strategy would be to control glucagon production with small molecules. In pursuit of this goal, we developed a homogeneous AlphaScreen assay for measuring glucagon in cell culture media and used this in a high-throughput screen to discover synthetic compounds that inhibited glucagon secretion from an alpha cell-like cell line. Some of these compounds inhibited transcription of the glucagon gene.
Palabras clave
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1
Base de datos:
MEDLINE
Asunto principal:
Glucagón
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Células Secretoras de Glucagón
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Bibliotecas de Moléculas Pequeñas
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Hipoglucemiantes
Idioma:
En
Revista:
J Biomol Screen
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article